Sentence examples for orientation of sRNA from inspiring English sources

dGenomic orientation of sRNA transcripts.

Strand orientation of sRNAs was also included in our analysis because abundant antisense siRNAs are known to be associated with repeats [ 32] and are affected by the loss of RNAi machinery [ 5].

a sRNA candidates, 5' and 3' ends predicted using SIPHT [ 35]. b The orientation of the sRNA and its flanking genes (in order of gene numbers).

c The orientation of the sRNA and its flanking genes (in order of gene numbers).

Moreover, we confirmed the orientation of the sRNA genes by employing single gene-specific primers for the reverse transcription reaction.

We thank Zonglin Hu for performing the tiled microarray that defined the orientations of the sRNAs.

fThe orientation of the flanking genes and sRNA gene (middle).

The number of sRNA copies was different in each cluster and were more abundant in sense orientation compared to antisense orientation.

Sequences of sRNA were then mapped to a Sorghum bicolor genome (JGI, v1.0) to find clusters of sRNA that matched the following criteria: i) clusters must contain no more than four non-overlapping sRNAs; ii) each sRNA in a cluster must be no more than 200 nt from its neighbor; iii) at least 90%% of sRNAs in a cluster must be in the same orientation.

Summary of sRNA sequencing data.

Classification of sRNA candidates in putative trans-encoded sRNAs, cis-encoded antisense sRNAs, mRNA leader transcripts, and sense sRNAs overlapping coding regions is ambiguous.