The red arrows show pFind1 insertion sites; the black arrows show the orientation of pFind1 at integration sites.
Overall, the important parameters that control the FRET systems are excited-state life time, medium refractive index, quantum yield of donor, relative orientation of donor acceptor dipole, integration of spectrum overlap between the donor and the acceptor, and the number of acceptors attached at each QD.
There are several possible causes for this variability, namely copy number of the transgene, site of integration, orientation of transgenic copies and transgene instability.
A significant difference was instead observed in the orientation of the intragenic integrations between the two datasets.
Crystal orientation and integration of reflexions were performed with MOSFLM [72] and scaling and merging of reflections with SCALA and TRUNCATE [73].
Since we have reported before that at some sites of integration, the orientation of the cassette plays a major role on transgene expression [37], we used PCR to characterize the orientation of the cassette in each clone and then selected clones with the cassette in each orientation for further analysis.
Essential features of the 5'-CS are the integrase gene (intI1), the product of which mediates orientation specific integration and excision of gene cassettes [6], a promoter region, containing the potential promoter, Pc to allow expression of the inserted cassette genes [7], and an integration site (attI1) for the site-specific insertion of gene cassettes [8].
We looked at Alu elements in sense and anti-sense orientation as depending on the strand of integration either one of the two possible orientations can manifest in a transcript.
Furthermore, four organizational antecedents have an impact on a company's network competence: access to resources, network orientation of human resource management, integration of intraorganizational communication, and openness of corporate culture.
