Sentence examples for orientation of insert from inspiring English sources

The presence and orientation of insert were confirmed by PCR.

The resulting clones were screened by PCR for the presence and orientation of insert.

A stock with isolation number 6.1 was selected for correct orientation of insert pBSattBattPLoxFRTy.

Depending on the transgene and orientation of insert, thoracic bristles may also acquire yellow+ pigmentation (D. Gohl and M. Müller, unpublished data).

Orientation of insert DNA post-recombination is dependent on the orientation of the sites prior to the reaction, with sites in the same orientation on a given DNA strand mediating excision of intervening sequence and sites in opposite orientation mediating inversion of intervening sequence.

The correct orientation of inserts was verified by sequencing.

Sequences and orientation of inserts in both constructs were verified by sequencing (GATC Biotech, Germany).

The reporter construct was sequenced to verify number and orientation of inserted oligonucleotides.

Orientation of inserts can also be determined using an internal primer.

An additional feature of this method is the capacity of determining the orientation of inserts, using an internal primer based on the insert sequence.

We have also used the Lig-PCR method to determine the relative orientation of inserts in recombinants generated from the above ligation reactions.