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The tunica corpus analysis emphasizes the orientation of division planes, but apices can be examined from other points of view the sizes of cells, the degree of vacuolation, and the concentration of various cell constituents, especially ribonucleic acid (RNA), vary through the apex and this sometimes results in more or less distinctive zones.
Another aspect of division geometry is the orientation of division axes relative to the embryo's spatial coordinates.
Finally, there is clear clustering of cells along the x-axis, suggesting that the orientation of division at the eight-cell stage has reasonable predicative power (Fig. 5A).
It is believed that the orientation of division of polarised blastomeres in the 8- and 16-cell stage embryo determines the fate of daughter cells, based on how asymmetrically distributed lineage determinants are segregated.
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In fact, as we describe in detail in Material and Methods and the relevant figure legends, all of our imaging sets, live and fixed, were 3D stacks, and therefore our tracing of the orientation of divisions is valid.
The method we used is not dependent on these parameters, as we determined the orientation of divisions by following the actual trajectory of the cleavage plane, in high-resolution and comprehensive 3D stacks of confocal images of dividing progenitors that are in an advanced cytokinesis stage, as we describe in detail in Material and Methods.
The functional megaspore cell undergoes the first mitotic division; the orientation of the division is longitudinal (Fig. 2D).
It can be seen that all division rules using the Random mechanism for the orientation of the division center generate distributions of number of neighbors that were wider than the one for the experimental data.
The relationship between cell polarity and orientation of cell division determines whether a neural stem cell undergoes symmetric or asymmetric cell division during development.
Apparently, the orientation of the division plane within the niche is responsible for maintaining this diversity; planar division generates two identical daughter cells, while apical-basal division generates asymmetric cell fates.
In many tissues orientation of cell division is coupled to the regulation of differentiation producing daughters with similar (symmetric cell division, SCD) or differential fate (asymmetric cell division, ACD).
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