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The orientation of cleavage was calculated based on the alignment of chromosomes stained with DAPI or TO-PRO-3 iodide as described previously (Sanada and Tsai, 2005).
The orientation of cleavage was calculated based on the alignment of chromosomes stained with H&E as described previously (Sanada and Tsai, 2005).
Yen1/GEN1, Mus81-Mms4/EME1 Mus81-Mms4/EME1ll shand the common property that HJ resolution gives rise to allixture of COshare NCOs, depending on the orientation of commonge (Fig. 1).
Improvements in microscope performance and the use of green fluorescent protein (GFP) now enable us to assign the orientation of cleavage planes in series of 3-dimensional images at far better spatial and temporal resolutions.
Depending on the orientation of cleavage, this might result in a crossover event, which could account for the large-scale sequence exchanges that can be demonstrated in meiotic cells.
The 'cell-polarity model' suggests rather that the orientation of cleavage of polarised blastomeres at both the eight- and 16-cell stages determines the fate of their daughters, depending on the equal or unequal partitioning of lineage determinants distributed asymmetrically along the apicobasolateral axis of the mother blastomere (Johnson and Ziomek, 1981).
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With regard to APs and BPs, the orientation of the cleavage plane could be deduced from the Tis21-nucGFP fluorescence, which outlined the cleavage furrow and revealed the position of the nascent daughter cell nuclei.
The precise position and orientation of Drosha cleavage is critically important for the generation of mature miRNA because the second cleavage step occurs at a defined distance from the free end generated by Drosha.
In mitotic cells, COs are strongly suppressed by tightly modulating the orientation of dHJ cleavage.
For apical progenitors, the orientation of the cleavage plane relative to their apical-basal axis is thought to be of critical importance for the fate of the daughter cells.
In APs, the orientation of the cleavage plane parallel to their apical-basal axis acts in concert with the polarized subcellular localization of cell fate determinants along this axis, thereby ensuring their equal distribution to the daughter cells on symmetric division, and a small deviation of the cleavage plane from this orientation suffices for asymmetric division to occur [14], [21], [22].
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