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These oriented molecular chains form the orientation nuclei which have higher thermal stability than the kebab-like lamellae that are developed later.
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Sapphire substrate surfaces have a specific epitaxial orientation (c- and a-axis), resulting in aspect-oriented nuclei.
For orientation cell nuclei were stained with 1% methylgreen.
a. Polarized growth in Matrigel unlike breast cancer cell lines propagated in a three-dimensional matrix (which develop apolar colonies with random orientation of nuclei and the basement membrane protein, alpha-6 integrin), HRBEC colonies were distinctive.
UCP2 knockdown (KD) in grade 3 cells resulted in the restoration of a polarized pattern of colony growth, reflected as basal redistribution of α-6 integrin and acini-like orientation of nuclei.
Non-malignant epithelial characteristics of HRBEC cultures included: a. Polarized growth in Matrigel unlike breast cancer cell lines propagated in a three-dimensional matrix (which develop apolar colonies with random orientation of nuclei and the basement membrane protein, alpha-6 integrin), HRBEC colonies were distinctive.
Note, Figure 1 depicts lung tissue from transgenic mice where columnar epithelium, typical for bronchioles with vertically oriented nucleus is replaced by cells displaying horizontal orientation.
Figure 2. Relative counting rates for particles from the decay of oriented 60Co nuclei for different nuclear orientations (field directions).
The orientation of recrystallized nuclei is selected between the orientation of the deformed grain and the orientation of the grain boundary area.
The annealing texture above 630 °C was very weak and determined by the orientation of recrystallization nuclei, whereas after annealing at 560 °C, the α-fiber (〈1 1 0〉//RD) character of the texture was enhanced, apparently as a consequence of selective growth of grains with α-fiber orientations.
We have added representative pictures and have highlighted that cell alignment was quantified by measuring nuclei orientation.
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