To tease out gravity's role, Ishay's hornets have even boldly gone where no hornet has gone before: In 1992, 180 of them were sent aboard the space shuttle Endeavor to see if microgravity would scupper the orientation and integrity of combs.
Orientation and integrity of the insert were confirmed by restriction analysis and sequencing.
The two constructs were sequenced to confirm the allele, the orientation and integrity of each insert.
All reporter constructs were sequenced to confirm the sequence, orientation and integrity of each insert.
The correct orientation and integrity of all constructs was verified by DNA-sequencing, performed by Eurofins MWG Biotech.
The orientation and integrity of cDNA inserts in the newly constructed plasmids were confirmed by automated DNA sequencing in the core laboratory of the University of Maryland School of Medicine.
The ER-β insert was subsequently cleaved from the original vector and sub-cloned into the pSG5 vector with orientation and integrity of inserts confirmed by sequencing [ 25].
Realisation of such concept, however, requires developing new methods for the formation of neural constructs ex vivo and, in particular, solving technical difficulties related to implanting such a construct or piece of tissue with orientation and integrity.
Orientation and integrity of the 1,405-bp 1,405-bpas confinsertby sequencing using the BigDye Terminator v3.1 Cycle Sequencing kits and the 3730 DNA Analyzer (Applied Biosystems, Foster City, CA) in three sequencing reactions using primers, Seq-S, Seq-A, and 3' NGSP-S.
DTI is a useful tool for examining and quantifying white matter microstructure, including the orientation and integrity of white matter tracts, by detecting the diffusion of water in neural tissue in vivo[ 10].
