Sentence examples for orientation insert from inspiring English sources

This tool integrates read quality, mate-pair orientation, insert size, coverage and k-mer frequencies to provide a statistical measurement of assembly quality.

The synthetic fragment also included a self-splicing td intron in the antisense orientation inserted between codons 15 and 16 of the kanamycin gene and cloned into pRB373.

After confirming insertion with the correct orientation, the insert was cloned back into the pPrPHG backbone using the PmeI/NheI sites.

The presence and orientation of insert were confirmed by PCR.

The resulting clones were screened by PCR for the presence and orientation of insert.

A stock with isolation number 6.1 was selected for correct orientation of insert pBSattBattPLoxFRTy.

The PCR product was digested with KpnI and XhoI enzymes and cloned into PGL3-basic luciferase vector and sequenced to confirm the orientation and insert (Supplementary Figure 2).

Depending on the transgene and orientation of insert, thoracic bristles may also acquire yellow+ pigmentation (D. Gohl and M. Müller, unpublished data).

Across all SSPACE assemblies only 1 1.8% of the MP read pairs satisfied the perfect match, read orientation, and insert size criteria (Table  3).

With a priori information from paired-ends such as order, orientation and insert size of pairs as constraints during read alignment to the reference genome, anomalously mapped pairs indicate potential genomic variations from the reference.

Orientation of insert DNA post-recombination is dependent on the orientation of the sites prior to the reaction, with sites in the same orientation on a given DNA strand mediating excision of intervening sequence and sites in opposite orientation mediating inversion of intervening sequence.