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Local orientation measurements and orientation images of grains nucleating at shear bands have been made using the electron back-scatter diffraction (EBSD) technique.
Having annotated eyes in the frontal dataset and nose and chin in the profile dataset, we perform the geometric normalization as described below [38, 39]: Normalization for 0° orientation: Images are automatically normalized thanks to the following steps: 1.
Figures 2(G)– 2(I) show averaged intensity, retardation and optic axis orientation images.
The retardation and axis orientation images show areas of varying retardation and axis orientation within epidermis and dermis.
The calculation time is ~200 seconds for a 3D data set of 1024 × 256 A-scans (which includes reflectivity, retardation, and axis orientation images).
The light directly backscattered by the SC is in a random polarization state (i.e., depolarized), which is clearly observed by the random color distribution of pixels within this layer in the retardation and axis orientation images.
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All data were processed by orientation image micrograph (OIM) patterns.
The specimens were analyzed using Orientation Distribution Function (ODF), optical microscopy and OIM (Orientation Imaging Microscopy).
c Orientation image indicating the angulation of vessels (scale represents degrees from 0 to 180).
The local crystal orientation is measured by orientation image microscopy analyses, again taken before and after the deformation step.
Grain orientation image maps (OIMs) were obtained for in situ crystallographic microstructural analysis of the test volumes.
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