Overall, the present study conclusively establishes the positive impact of the substrate conductivity towards cell proliferation and differentiation as well as confirms the efficacy of HA-CaTiO3 biocomposites as conductive platforms to facilitate the growth, orientation and fusion of myoblasts, even when cultured in the absence of external electric field.
Activity towards CMC clearly indicates the functionality of endoglucanase, when produced as single protein and in both orientations as fusion enzyme.
These fusion orientations mimic the orientation of the nuclease domain to the DNA-binding domain in the native enzymes (Derbyshire et al. 1997; Li et al. 1992).
From this analysis, ChimeraScan performed better than all the other tools concerning the number of detected chimeras and the correct orientation of the fusion events; 19 out of 27 fusions were all detected in the right orientation.
Due to the competitive growth, both the ferrite and austenite grains showed almost the same < 001 > ║ ND orientation near the fusion boundary.
It was found that the magnetic properties of the welded area experience significant decrease due to the formation of large grains that have unfavorable orientation in the fusion zone.
Thus, LIF2 is targeted to the nucleus, but LIF2 protein can also be localized in the cytoplasm, possibly depending on the fusion orientation of the fluorescent marker, on the host type cells or the transformation technique.
These results showed that the orientation of the fusion proteins was important and suggested that the N-terminal part of CRX, where the DBD is located, had to be accessible for efficient NR2E3/CRX interactions.
Moreover, RsSGD was localised to the nucleus with the same fluorescence pattern as CrSGD according to the orientation of the fusion in agreement with the predicted C-terminal bipartite NLS (514-A KR RREEAQVEL V KR Q K T-532).
The orientation and exact fusion points between HSA 10 and 11 in both taxa need to be tested with BAC FISH and sequencing to determine if this syntenic association is equivalent or different.
