Template M1 features a central tetrahedral centre bearing a potential Mg2+-interacting carboxylate group plus two heteroaromatic branches that have dimensions and relative orientations designed to fit snugly into the active site groove.
A PCR experiment using primers with opposite orientations designed to amplify solely TIR-220 repeats resulted in a "ladder-like" pattern of amplicons representing multimers (up to seven) of approximately 220 bp, confirming the tandem organization of TIR-220 repeats seen in the assembled genome of D. virilis (supplementary fig. S3, Supplementary Material online).
In this procedure, the result for each step provides the isotropic design, continuous fiber orientation design, and functionally graded discrete orientation design, respectively.
To avoid intermediate fiber orientations and achieve discrete fiber orientation design, a penalization scheme is applied to the orientation design variable.
The orientation design variable controls not only the fiber orientation, but also fiber volume fraction.
Increasing the canalization orientation design gives a better focused spray.
In this method, an orientation design variable in the Cartesian coordinate system is employed with a conventional density design variable.
The fiber volume fraction control can be used to relax the orientation design problem and simultaneously design a functionally graded structural layout of fiber material.
To solve this problem, a new two-arc orientation design model is proposed that meets the orientation requirements and is easy to operate on site.
The paper argues that, despite its process orientation, design methodology cannot avoid addressing questions concerning the nature of the products being designed for two reasons.
In the DSA, a material derivative is utilized and the kinematical description of GEBT is consistently employed to express orientation design variations.
