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A PCR fragment that had an approximate length of 700 bp was detected with the pb-3 forward primer (5'-AGGATTATGGGTCAAGTTCAAGGAAG-3') and pb-1 reverse primer (5'-CTTATGGACTCTTCCTGCAGCAGAT-3'), indicating that pb-3 is located upstream of pb-1 with a 613 bp interspace region in the same orientation (data not shown).
The activity of the CNS2 did not depend on its orientation (data not shown).
No metabolite of alprazolam was detected in cells transfected with vector alone or vector containing the cDNA in reverse orientation (data not shown).
We also observed that OCT4 and SOX2 motifs tend to have a closer distance between each other, independent of strand orientation (data not shown).
We also tested the "inverted" Met31 model alone, and inverted Met31 upstream of Cbf1, but the results were similar to equivalent scans with the "normal" orientation (data not shown).
However, expression of Wg along the ventral midline in this mutant had no observable effect on denticle orientation (data not shown) likely due to overall low protein levels [31].
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Surprisingly, injection of mRNAs encoding CENPB-eGFP-MCAK instead of Sgol2 did not accelerate chromosome bi-orientation (data not shown), raising the possibility that Sgol2 facilitates bi-orientation by a mechanism either independent of or in addition to MCAK's recruitment to kinetochores.
To facilitate the comparative analysis of different conserved sequences from different enhancers, EvoPrinterHD allows for the curation of CSBs by enabling the user to automatically extract and collate CSBs in both forward and reverse-complimented orientations (data not shown).
We found a significant positive correlation between the surround MI in the attend away condition and the attention MI when a surround was present (P < 0.001, r = 0.429, Spearman rank correlation, Fig. 5B preferred orientation responses; P < 0.001, r = 0.309, responses to all bar <span class="lh lhl">orientations, data not shown).
Moreover, surround MIs (measured when attention was directed to the RF) were positively correlated with attention MIs measured in the absence of surround stimuli (P = 0.002, r = 0.305, Spearman rank correlation, Fig. 5C preferred orientation responses; r = 0.334, P < 0.001, responses to all bar <span class="lh lhl">orientations, data not shown).
No correlation between surround MIs measured in the attend RF condition and attention MIs measured when surround stimuli were present was found (P = 0.991, r = −0.001, preferred orientation response MIs, Fig. 5D; P = 0.819, r = −0.023, MIs from responses to all bar orientations, data not shown, Spearman rank correlation).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com