For TFBSs, their distances to TSSs, their orientations, copy numbers and the interaction between any two adopted TFBSs are all taken into account.
For each tile the following tasks are executed: All the images corresponding to the processed tile as well as their orientation are copied to the machine where the tile is processed.
For this analysis, modules, or discrete regions of high identity, were concatenated when they were <5 kb apart or separated by repetitive elements, as long as the modules maintained their orientations between copies.
There are several possible causes for this variability, namely copy number of the transgene, site of integration, orientation of transgenic copies and transgene instability.
Primers were designed to amplify both in a typical inward direction, and in a reverse orientation between gene copies using the Primer3 software in batch mode (table of primers available on request).
Column 7 – orientation of the copy.
However, the current pig assembly shows two copies of MITF, and most of the predicted promoters based upon human promoter mapping lie downstream and in the opposite orientation to both copies.
Altogether, this panel allows the user to examine the relative location (distance and orientation) of TE copies and genes, and, therefore, may indicate possible roles of TEs in gene regulation.
From these nine phages we have isolated and described four hsp70 copies in tandem orientation hsp70S2-S55) and a fifth copy in inverse orientation (hsp70S1).
Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene.
In this study, we used a validated Chinese version of the Mini-Mental State Examination (MMSE) which assesses orientation, memory, visual-spatial copying, and language.
