After orientation was marked, the eyes were fixed overnight in 2.5% gluteraldehyde prepared in 0.1 M cacodylate buffer and processed as prescribed previously [20].
The different types of facet planes present in each orientation map are marked schematically by hexagons ((119),~8°), squares ((114),~20°), triangles ((113),~25°) and open circles ((112),~35°); only one from each type is marked.
The particular resonances in Figure 5(a), well resolved for some intermediate orientation angles, are marked as I, IIa, and IIb. Figure 5(b) presents the related dependences of the measured longitudinal relaxation rates (T_{1}^{-1}) on the B-field orientation at room temperature.
These organizations, all vivid examples of the sales machine, are marked by a strong process orientation, clear lines of authority, and close governance through formal rules.
Nevertheless, additional needle orientations, which are present in minor fraction, can be observed and are marked by small red (B2) and blue (B1*) arrows, respectively.
To maintain orientation, tumor margins were marked on the corresponding H&E-stained slide.
The orientation of the eyes was marked on the limbus at 12 O'Clock position before enucleating.
The samples were attached to paraffin tablets by sterile surgical needles; the orientation of the mucosa was marked on the tablet (anterior and posterior sides) and sent to the Institute of Pathology under sterile conditions for further processing.
Eyes were marked for orientation, then enucleated and immersion fixed in 2.5% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.3, at 4°C overnight.
Eyes were marked for orientation, then enucleated, punctured behind the cornea for better penetration of the fixative, and immersion fixed in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB, pH 7.4) for 2 3 hrs (opsin immunocytochemistry) or several days (Nissl staining of ganglion cells).
