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Changes in cytoskeletal organization were assessed by immunofluorescence staining of the actin fiber network.
To this end, SCP2 cells transfected with either Scr or cyclin D1 siRNAs were stimulated with TGFβ and the dynamics of actin organization were assessed by staining with the fluorescently labeled F-actin marker phalloidin and mesenchymal intermediate filament vimentin.
The cellular infiltrate and the lymphoid organization were assessed by immunofluorescence staining of sequential sections with monoclonal antibodies recognizing CD3, CD20, CD21 (all provided by Dako Cytomation, Glostrup, Denmark), CXCR4, CXCL12, CXCR5, and CXCR13 (all provided by R&D System, Abingdon, UK).
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Table 5 shows how each organization was assessed.
Domain organization was assessed using the conserved domain database CDD [34] with a cutoff of 0.003.
Local spatial organization was assessed through the value similarity between each polygon and its nearest neighbors.
The spatial organization was assessed by comparing the location of class profiles on the SOM to a dendrogram created by applying Ward's hierarchical clustering to the SOM class profiles [ 26].
Terry Backer, the Long Island Soundkeeper, said his organization was assessing the situation on Hen Island and was considering whether to join Mr. Tartaglione's lawsuit.
Regional Executive Officer Barry Porter said the organization is assessing whether it is prepared for another storm after utilizing so many of its resources for Harvey.
The organization is assessing the situation and has identified the greatest needs as food, shelter and water, as well as gloves and machinery for rescue workers.
The organization is assessing the best path for distributing funds directly to those affected, victims' families and to local nonprofits providing critical services, the CDF President Bob Ottenhoff told HuffPost.
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