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SEM has also provided details of the collagen organization in normal, degenerated, and repaired human articular cartilage [ 52].
To learn about the role of the cytoskeleton in CAFs, we compared its organization in normal fibroblasts (NFs) and CAFs from the MMTV-PyMT mammary tumor model (Calvo et al., 2013).
Proteins involved in the organization and regulation of the cytoskeleton, including Kinesin HSET/Ncd (which is not required for polar organization in normal somatic cells) and the four chromosomal passenger complex (CPC) components, Aurora-B, INCENP, Survivin, and Borealin were identified among the main proteins required for bifocal clustering of extra-centrosomes in these cells [ 12, 17].
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The organization, based in Normal, Ill., not only conducts the big annual Bloomington Gold show but also sets the standards for judging how faithfully the entries match factory specifications.
Here, we use nanoscale-resolution TEM to study the chromatin organization in microscopically normal-appearing cell nuclei that may be undergoing early-stage tumorigenic alterations.
Similar (re)organization was detected in normal males and thus this was not attributed to the effect of NBR.
For the efficient target ALS2CL, miR-449b repressed it only in CIN I stage, and it had influence on the 'endosome organization' process only in normal stage.
By moving a magnetic bar underneath free flowing bead suspensions in culture media, followed by in situ gelation, it was possible to form patterned gels in layers in partial mimicry of the organization of cells in normal tissue.
This chapter focuses on the three-dimensional organization of the nucleus in normal, early genomically unstable, and tumor cells.
In summary, the characterization of NPC deterioration at the molecular level might uncover molecular mechanisms that induce or contribute to global changes in genome organization and gene expression in normal and pathological aging.
Similarly, when Xenopus S3 cells were injected with dynamitin, we observed no changes in spindle pole organization by GFP tubulin imaging in normal cells containing bipolar spindles or in cells with unseparated spindle poles that had been preincubated with monastrol.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com