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The organisms were searched in PATRIC database to check their host and pathogenicity.
Specific organisms were searched for Gram negative bacteria: Acinetobacter, Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, and for Gram positive bacteria: Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Enterococci (VRE) and Clostridium difficile (C. difficile).
In order to analyse conservation of the APITD1 gene, the TIGR gene indices (URL: http://www.tigr.org), which contain genomic sequences from a variety of organisms, were searched for expressed sequences translated in all reading frames, with significant similarity to the predicted protein from APITD1.
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Genes are searched for abbreviation, name, EC number, KEGG ID, and UniProt ID Organisms are searched for name.
Spectra derived from a single condition for each organism were searched against the genome sequences of progressively more genetically distant (based on 16S-rDNA sequences) neighboring organisms.
All the T6SS orthologs identified in each organism were searched to find interacting partners among them in order to identify components of the network that had been missed out by sequence similarity based methods.
In this study, we employed a systematic peptide identification strategy in which spectra derived from one organism were searched against the genome sequences of progressively more genetically distant neighbor organisms to measure the extent to which proteomic information could be obtained about one species when using the genomic sequence of another.
Gene orthologs of each candidate gene for each organism were searched and collected from NCBI Entrez gene database (Human-NCBI 36.2, Mouse-NCBI M37.1, Rat-RGSC v3.4) [ 32].
Then, the best hit of each organism was searched against the human proteome and sequences not re-identifying the starting sequence were removed.
The genomes of the organisms listed were searched for the two conventional CCCH motifs and in addition for two fantasy CCCH motifs (C-X7-C-X7-C-X3-H and C-X8-C-X7-C-X3-H).
To identify the chaperonins in the microbial organisms, chaperonin homologs were searched for using BLAST (e-value 1*10-4) against a chaperonin database cpnDB downloaded on June 2011.
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