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Bacterial organisms were passaged on tryptic soy agar (TSA) (Oxoid, Toronto, Canada) medium at 35°C aerobically for 24 to 48 hours.
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Cells were passaged with Trypsin.
Four were passaged further (17.4%).
Cells were passaged twice weekly.
RASF were passaged for up to 8 passages.
Cells were passaged by trypsinisation.
Cells were passaged when confluent.
The infecting organisms were previously passaged through infant rats in the course of experimental studies [ 9].
Some organisms are then passaged to the next stage in the tick life cycle and can be transmissible during the subsequent blood meal (5 ).
The organisms were maintained by serial passages in mice until required.
The organisms were maintained by serial passages in mice until required as used and described by different workers [ 15, 16].
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