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To a background of DNA generated from natural outdoor aerosols, known quantities of rRNA gene copies from distinct organisms were added producing corresponding hybridization intensity scores that correlated well with their concentrations (r = 0.917).
Then, anaerobic organisms were added to leachate at ratio of 1 10 (150 mL of anaerobic organism) using measuring cylinder.
Briefly, the second stage suspension cultures (25 mL) of all experimental organisms were added with racemic Carvedilol solution in methanol (0.01 mg/mL culture).
The cultured organisms were added in 10 ml saline solution to reach the concentration of bacteria to 108 colony forming units per milliliter (CFU/ml) corresponding to MacFarland scale.
Proteomes of seven fully sequenced organisms were added to the analysis.
When these two organisms were added to the flow cell system, each organism initially formed small microcolonies.
Similar(50)
The core oligosaccharide added onto nascent chains has the same composition and in both organisms is added onto Asn-Xxx-Ser/Thr glycosylation sequons.
A suspension of the organisms was added to sterile nutrient agar media at 45 °C and the mixture was transferred to sterile Petri dishes and allowed to solidify.
Test samples (10 mg/mL as stock solution in DMSO and serial dilutions) were transferred to 96-well clear plate in triplicate, and the suspension of the test organisms was added to each well achieving a final volume of 200 μL (ampicillin and fluconazole were used as positive controls).
Cycloheximide (CHX, Sigma-Aldrich, Inc ., an inhibitor of protein synthesis in eukaryotic organisms, was added to the medium 36 hrs after transfection at 50 ug/ml final concentration.
A loopful of organisms was added to 18 mL sterile 0.2 M phosphate buffer in 0.9% NaCl, pH 7.4.
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