Your English writing platform
Free sign upSuggestions(1)
Exact(12)
Other researchers are pursuing metagenomics, in which the goal is to comprehensively profile all of the organisms in a sample.
Such an analysis involves grinding up all organisms in a sample, sequencing all the genes and then matching each with a unique microbial species.
The index of alpha-diversity summarizes the diversity of organisms in a sample and is estimated according to the distribution of species-level annotations.
The team also carried out a so-called metagenomic analysis, in which the genomes from all organisms in a sample are sequenced collectively; the great majority of genes they found coded for proteins never seen before.
Thus, in principle, one can use PCR to amplify the rRNA genes from all organisms in a sample in a culture-independent manner.
A fundamental challenge in metagenomics has been estimating the abundance of organisms in a sample based on the frequency with which the organism's DNA was observed in reads generated via DNA sequencing.
Similar(48)
Conventional qPCR is designed to enumerate the gene copies of an organism in a sample, but does not identify those that originated from a viable pathogen.
N = Total number of an individual organism in a sample.
They are optimized for forensic use, to identify the specific strain of organism in a sample as precisely as possible.
Srinivasan et al. [ 14] developed an alignment-free n-gram-based method named MetaID that can accurately identify microorganisms at the strain level and estimate the abundance of each organism in a sample given a metagenomic sequencing dataset.
Here, we present MetaID, an alignment-free n-gram based approach that can accurately identify microorganisms at the strain level and estimate the abundance of each organism in a sample, given a metagenomic sequencing dataset.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com