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Since this method is so simple, general, and yet powerful, it can be applied to various organisms and cells, especially single-celled, uncluturable ones, for their genome analysis.
Two realms were soon revealed: the macrocosmic world of celestial bodies and the vast distances between them, and the intimate, interior world of microscopic organisms and cells.
Reductionism is the scientific practice of trying to understand complex objects like organisms and cells by breaking them down into their smallest components, a method that critics complain devalues the whole.
The damage produced by UV-C radiation (100 280nm) in organisms and cells is a well known fact.
These targets can be selected using prior knowledge of disease-associated pathways or identified using phenotypic chemical and genetic screens in model organisms and cells.
Zinc-finger nucleases are one of a few genome-editing tools that researchers use to create specific changes to the genomes of living organisms and cells (see "Genome Surgery").
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(BOSTON) — The CRISPR-Cas9 system has come to be known as the quintessential tool that allows researchers to edit the DNA sequences of many organisms and cell types.
Nonhomologous end joining (NHEJ) is a mechanism of DNA double-strand break repair in nearly all organisms and cell types.
Most eukaryotic species have two distinct condensin complexes whose balanced usage is adapted flexibly to different organisms and cell types.
Herein we discuss the recent development of CRISPR-Cas9 into a key technology for genome editing, targeting, and regulation in a wide range of organisms and cell types.
The fact that ZFNs and TALENs have been used for genome modification of more than 40 different organisms and cell types attests to the success of protein engineering.
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