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Short sequence reads present a confounding problem of multiple organisms' alignment (Trapnell et al., 2009a) and thus inconclusive identification of the organisms.
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The active site architecture of GGTase-II (β-subunit) of L. donovani was compared with other organisms by alignment.
Orthologous sequences are often inferred across organisms by alignment of two or more sequences and the nucleotide sequence similarity between them.
Nearly all of the detected non-vertebrate organisms had alignments from multiple tissues, which would be expected for blood-borne parasites.
With the help of organism specific alignments, sequence and gene context analysis, it could be confirmed that SafA does not only occur in E. coli but also in Shigella and Salmonella.
Such homologous proteins can be efficiently identified in distantly related organisms by sequence alignment.
Thus, we compared the H. pylori proteome to a set of known proteases from various organisms using sequence alignment techniques.
Due to sequence conservation of functional genes and the rapidly growing molecular knowledge of model organisms, basic local alignment search tools (e.g. BLAST) facilitate the initial annotation of non-model genomes [ 2].
This module explores topics in elementary graph theory, linear algebra, modern and polynomial algebra, combinatorics, linear programming, and algebraic statistics through applications to a central and profound problem in biology: the determination of hereditary relationships among organisms through the alignment of DNA sequences from creatures currently in existence.
The same recommendation is likely to apply to other organisms and other alignment-ambiguous loci.
Another concern is the heterogeneous distribution of the evolutionary distances between the organisms in the alignments.
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