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To further improve the organic stability of the enzyme, Kartal et al. (2011) suggested the mixing of acetone with another buffer.
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Together these results indicated that, in contrast to the organic solvent stability of some lipases [ 21], which had no relationship with the polarity of the organic solvents, the stability of the esterase from strain LY9 was probably dependent on the polarity of the solvents, which increased only in non-polar organic solvents with higher log Pow values.
Fig. 4 Organic solvent stability of CLEA-protease.
The simulation results clearly showed the importance of organic matter stability and growing site design.
Among the candidate mutants, the A8T, A92E, N97Q and T245S mutants showed higher organic solvent stability at various methanol concentrations.
Several methods have already been successfully applied to differentiate soil aggregates by their origin, but they cannot specify the degree of organic matter stability within soil aggregates.
Moreover, the nanofibrous enzyme expressed excellent organic solvent stability; approximately 70% of its initial activity was retained after incubation in xylene solvent for 24 h at 25 °C.
Furthermore, the formation of salt bridges and hydrogen bonds led to improved organic solvent stability of the lipase (Pseudomonas aeruginosa LST-03) in presence of organic solvents (Kawata and Ogino 2010).
They have reported that the disulfide bonds and amino acid residues located on the surface of the molecule play an important role in the organic solvent stability of the enzymes.
These studies indicate that organic solvent stability is a general property of halophilic enzymes, owing to their ability to work at low water activity.
32d– f Here we briefly mention two studies with a focus on thermal stability: organic semiconductors able to withstand autoclaving; 33a and organic semiconductors with a thermal stability up to 250 °C.
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