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At least six sample pools containing the most abundant taxonomic orders were analysed per site and month.
The differences between gene orders were analysed using common intervals [ 38], breakpoints [ 64] and reversal distances [ 65] implemented in the CREx tool [ 38].
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When all orders are analysed together, the allometric slope for lactation duration is close to, but still shallower, than the slope predicted by the MTE (0.15 < b < 0.23), and the intercept is intermediate in value (11 < a < 31).
The allometric slope for gestation also falls below the 0.25 slope predicted by the MTE when the orders are analysed together (0.10 < b < 0.14).> The allometric slopes for lactation duration are more variable than those for gestation duration.
Branch order was analysed as shown in Fig. 2 (Coleman and Riesen, 1968).
First and higher-order kernels were computed but only the first and the second-order kernels were analysed.
Species abundance was aggregated into progressively higher taxa matrices (genus, family, order) and were analysed using multivariate techniques.
Gene order rearrangements were analysed on two datasets, with or without tRNA genes, and the extra genes of P. fumigata (trnI-2 and trnX) were excluded from all analyses.
Subjective symptoms, which are ordered paired data, were analysed using a nonparametric Wilcoxon signed-rank test.
Models including ordered categorical variables were analysed as a test for linear trend.
In particular, the responses to questionnaires on the Likert scale were treated as ordered categories and were analysed by the Mann–Whitney test (Altman, 1991).
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