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Among the three major archaea orders investigated (i.e., Methanosarcinales, Methanobacteriales, and Methanomicrobiales), Methanobacteriales were encountered at highest concentrations (1.9 × 1011 gene copies mL−1).
Analysis of available sequence data from other arthropoda (including cDNA, EST, protein and genomic data) suggests that the loss of selenoproteins has been confined to the infraclass Endopterygota, affecting species of all orders investigated (Hymenoptera, Lepidoptera, Diptera and Coleoptera).
In summary, existing data do not support selenoprotein loss outside the Endopterygota, but within this infraclass, the loss appears to be generalized across the orders investigated, with the exception of the Diptera.
All orders investigated in this study are labeled by an asterisk.
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