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These studies suggest an ordered pathway for the assembly of promoter chromatin architecture.
The latter will increase the surface area allowing for more QD loading and for an ordered pathway for faster electron transport.
It has been described as an ordered pathway, with initial cleavage by aspartic proteases followed by action of other proteases, eventually leading to free amino acids [12].
The series of events that drives auto-activation of PR and maturation of RT and IN also follows a sequential and ordered pathway, beginning with the p2-NC cleavage [40].
A key goal of the present study was to more rigorously test the hypothesis that falcipain-2 and falcipain-3 indeed hydrolyze hemoglobin through an ordered pathway of cleavage events and, in any event, to identify globin peptide and hemoglobin cleavage fragments after incubation with falcipains.
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Our results suggest that evolution has selected for protein complexes that assemble via well-defined, ordered pathways.
An important tool for ordering pathway components is genetic epistasis analysis, and here we present a strategy termed Alternative Inputs (AIs) to perform systematic epistasis analysis.
Thus, the ordering pathways for the volume-filling of metastable bcc-sphere are almost identical to that for the volume-filling equilibrium bcc-sphere.
We ordered each pathway with respect to their AUC and we found 32 paired pathways with AUROC > 0.90 between BC and NS.
We ordered each pathway with respect to their AUC and we got the first 10 pathways with the best 10 AUC.
Thus, the enzymes appear to degrade hemoglobin not via an ordered hydrolytic pathway, but through rapid hydrolysis of multiple sites.
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