Within each lineage, Pearson correlation (r) was used as a measure of co-expression between the ordered expression profiles of all expressed gene-pairs across the set of 20 samples (5 replicates per cell stage).
The differences between both approaches can also be seen by visualizing the ordered expression matrices (data not shown).
Note that all reference genes are selected on the basis of their rank ordered expression stability (Fig. 2A).
The differentiation of ESCs toward cardiac populations that include cardiomyocytes are characterized by a temporally and spatially ordered expression of specific genes.
These three genes have been proposed to define a central regulatory pathway that controls ordered expression of conidiation-specific genes during conidiophore development and spore maturation [5], [6], [11].
In wild type cells, genes involved in a metabolic shift to gluconeogenesis and various stress-resistance pathways exhibited an ordered expression profile timed with multicellular development even though they are not CsgD regulated.
