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Therefore, we conclude that the cross-hybridisations of wheat BAC pools with barley expression microarrays is a straight forward approach to order wheat contigs with gene-based markers without the difficulty of EST genetic mapping in wheat.
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I started ordering wheat, in fifty-pound buckets, from places in Montana and South Dakota.
Each company defined colors differently, and when you ordered "wheat" or "taupe" or "cream," you couldn't predict what you'd get.
The conservation of gene order among wheat homoeologous chromosome and across the grass family was exploited here to summarize diversity in the wheat genomes using a single map.
All Bobby wants is an omelet with an order of wheat toast, but inexplicably, it only comes with a roll or English muffin.
Comparison of gene order in wheat and Brachypodium sylvaticum, a wild pooid grass, has shown a high degree of conserved gene order and sequence similarity between orthologous regions of the wheat and Brachypodium genomes [5], [6], and genome sequences of Brachypodium have been used for the rapid and accurate assembly of a large contig of sequenced wheat BACs [6].
A virtual gene order of wheat chromosome 5D was constructed using the 'genome zipper' approach [ 8].
Thus, globally we expected the genes to be in the same order between wheat and barley but rearrangements are likely to be observed locally.
Here, we wanted to explore the possibility of using barley transcript genetic maps as a surrogate to anchor and order the wheat physical contigs.
As the relative order of wheat genes in a given deletion bin is not known, we inferred this order from the barley chromosome 3 H and rice chromosome 1 data.
In addition, comparison of the order of wheat ESTs and rice pseudomolecules suggested that at least two inversion events have occurred in this chromosome region besides duplications (data not shown).
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