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The map order was refined using the annealing and flips procedures of CarthaGene.
Marker order was refined using the "annealing 100 100 0.1 0.9" command of CarthaGene software.
Subsequently, gene order was refined using a procedure that maximizes the correlation between neighboring clusters while maintaining the same network of connections in the dendrogram.
The marker order was refined using RECORD [ 40] and the goodness-of-fit of the marker order was examined by plotting pairwise recombination fractions against LOD linkage statistics.
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14 This gross level of order is refined, with blocks of variants appearing within each locus category.
Once all markers from both data sets are placed, the marker order is refined iteratively, cycling through polish and flips routines until likelihood does not improve.
Then, the white matter surface was refined in order to obtain submillimeter accuracy in delineating the gray/white matter junction.
Note that the alignment was refined in order to properly position the predicted β-strand 10 of Drg1.
Subsequent to the completion of both experiments the applied terminology was refined in order to better characterize and group different types of gonad changes observed and which in turn could be analysed.
WHO step 1 (non-opioid analgesics) was refined in order to enable further and clinically relevant differentiation between non-opioid analgesics (level 1: paracetamol, level 2: prostaglandin inhibitors and level 3: anticonvulsants).
The Recombinase-mediated Cassette Exchange (RMCE) insertion protocol (Feng et al., 1999; Lienert et al., 2011) was refined in order to scale the needs of inserting large number of fragments in parallel.
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