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CLE files for the 24 diseases were collected independently and RMA normalization algorithm [27] was used in order to normalize data.
cGAPDH was used as a control gene to calculate ΔCt in order to normalize data.
Affymetrix.cel files were imported into Partek with the Robust Multichip Average algorithm (RMA; [48]) feature 'on' in order to normalize data sets.
In order to normalize data, arc sine, log10, and square root transformations of the data were performed and evaluated by number of variables normalized.
A set 'gain' for the plate reader was established for all scans in order to normalize data across numerous days/scans.
Similar(55)
Only the very last step of data analysis requires STIMT data, in order to normalize PIXET data to obtain concentration distributions, in terms of normalized mass fractions (in μg/g).
For analytic purposes, urine protein data were log transformed in order to normalize the data.
For comparisons of testis and deferent duct volume for sub-adult versus adult males, the data were natural-log transformed in order to normalize the data distributions, and analyzed using two-tailed t-tests.
The SCR data for each individual were square root transformed in order to normalize the data.
In order to normalize the data, specific planes were used as the basis for measurements.
In order to normalize the data for comparison purposes, we chose the same base cache hierarchy configuration defined previously.
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