The data obtained from this optimization process were used to build a second-order stepwise linear regression model [ 37] (Supplementary Methods) to determine the relative importance of the individual drugs.
Our report provides new insights into development of antifolate-resistant malaria and supports the findings of McCollum et al. (10 ) that the mutations in the dhfr gene do not always occur in a predictable, ordered, stepwise manner.
Construction of a large multi-subunit machine such as the proteasome is expected to require high precision [ 44]. 20S CP biogenesis is an ordered stepwise process requiring five dedicated chaperones that have been described in detail [ 45, 46].
By binding with low specificity to single-stranded RNA, CspA can prevent the formation of unwanted secondary structure and thereby narrow the RNA folding landscape (Jiang and Hou, 1997)—a mechanism of action reminiscent of the ubiquitous protein chaperone DnaK (Hsp70), which cycles on and off nascent polypeptide chains to allow ordered stepwise folding (Hartl et al., 2011).
Our data suggest that the order of stepwise exclusion, rather than the absolute M value, is a better index of the relative expression stability of individual candidate genes within a gene panel.
IGENT supports exhaustive search (IGENT_exhaustive) for lower-order interaction and stepwise search (IGENT_stepwise) for higher-order interaction.
These pumping rates are used in reverse order as a stepwise (monotonically increasing) pumping strategy.
In order to demonstrate stepwise baking without starch melting of cookie, the effects of water content on the starch melting temperature (Tm) and baking temperature on the water evaporation rate were investigated in cookie dough.
As DJ joints were not detected by Southern blotting, we deduced that the stepwise order of VDJ assembly for the TCRβDMF is mainly V-to-D rearrangement, followed by VD-to-J rearrangement.
The potential confounders were added to the model in a stepwise order according to their effect on the risk estimate but were kept in the model only if the exclusion changed risk estimate at least 5%.
The model building process was therefore carried out in the following order: A forward stepwise procedure with a test for backward elimination of covariates to obtain a preliminary main effects model.
