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The enzyme has a fixed order of substrate binding, with ATP binding first, followed by HMDP.
The enzyme deglycosylation induces significant changes in the order of substrate selectivity for gAmPDH and dgAmPDH.
Initial-rate kinetic experiments are then employed to reveal optimal reaction conditions, to evaluate the Michaelis constant, Km, and maximal velocity, Vm, to establish the order of substrate addition and product release, and to define the basic modes of inhibitor and activator action.
From these crystal structures, a sequential order of substrate binding was postulated.
To explore the mechanism of formation of the ternary complex, i.e. the order of substrate binding, two sets of experiments were designed.
Here, the order of substrate binding as well as the underlying conformational changes were investigated by NMR confirming the model derived from the crystal structures.
Similar(20)
The binding order of substrates to CaMKII has been investigated in the past.
For single-displacement reactions, the order of substrates binding to the enzyme can be random or ordered.
The presented model is targeted at predicting behaviours of highly flexible piezoelectric devices (FPEDs) and includes high orders of substrate and piezoelectric material nonlinearity, geometric nonlinearity, and additionally the effects of both self-weight and pre-stress.
This ordering of substrate modification allows a small number of master regulators to carry out their functions over a large time window with high temporal resolution, enabling precise and robust control of the numerous processes underlying cell cycle progression [ 1, 2].
When the order of titrating substrate was reversed, by titrating protein substrate first, non-specific signals were obtained indicating the lack of any detectable binding (data not shown).
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