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As shown by the EC50 values, the order of specificity in activating lfSCTR was hVIP (0.16 µM >hSCTAP27 (0.79 µM >xSCT (0.79 µM)>xSCT (1.82 µM).
We have ordered 52 pathways in the order of specificity with which they could be identified by Psi-square in this work.
As shown by their EC50 values, the order of specificity was hVIP (10.3 nM >hSCT (14.7 nM >xSCTAP273.15.6 nM)>xSCT (273.1 nM), which is in agreement with the cAMP assay.
Interestingly, the order of specificity changed from what we observed in titrating H3.
The order of specificity for p300 mimics that of CBP (K18 > K14 > K23).
For CBP, when titrating H3/H4 the order of specificity for H3 acetylation is K18 > K23 > K14.
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The order of the specificity constants for CBP is K14 ≈ K18 > K23, and the constants for K14 and K18, was ∼8-fold higher than K23.
This system closely mirrored the in vivo cortex, in terms of the temporal order of development, specificity of circuit formation, and laminar organisation of projection neurons (Shi et al., 2012).
Only about 1 out of 10 000 candidate mosaic sites reported by the GATK pooled-sample model were expected to be real, whereas our pipeline achieved order of magnitude higher specificity (Supplementary information, Figure S7).
The study confirms the relative ordering of the specificities for these six tests seen previously in a referral population (Szarewski et al, 2008, 2012), but now also in a screening context.
The order of reactivity and substrate specificity for dNDP is dADP > dGDP > dCDP > dTDP in the pyruvate kinase (PK) reactions.
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