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How cytokinetic nodes assemble, whether the order of assembly of ring components is important, and whether Mid1p solely participates in ring positioning are poorly understood.
Uniquely, the order of assembly can be predefined.
Uniquely, this method allows the order of assembly to be predefined.
It is to determine the order of assembly tasks to be performed sequentially.
HRSG construction varies widely in levels of modularization and order of assembly of the components.
An incorrect temporal order of assembly thus readily yields a non-native three-component ribonucleoprotein particle.
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An ordering of assemblies based on their average rank provides only minor differences to that of the z-score ranking (Additional file 2: Figure S10).
Their mutations, which do not alter RING folding, abolish PML NB assembly, demonstrating the role of tetramers in higher order of macromolecular assembly (Figs. 2 4).
Despite the self-assembly properties of most subunits it is clear that ESCRT-III filaments require a defined order of subunit assembly.
However the B12/23 restriction does not explain the order of TCRβ assembly for which the regulation remains an unresolved issue.
As DJ joints were not detected by Southern blotting, we deduced that the stepwise order of VDJ assembly for the TCRβDMF is mainly V-to-D rearrangement, followed by VD-to-J rearrangement.
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