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Subsequently, the BUILD option was used to order markers within each linkage group.
Finally, the marker positions and error parameters were established by order markers module.
The build option was used to order markers within the linkage group.
The marker order was obtained using the order markers module of Lep-MAP.
To order markers within linkage groups, we used the 'Order Markers' option of AntMap with default settings (Haldane mapping function), except allowing a greater number of runs (20 instead of 3) to confirm an optimal solution.
JoinMap4 (Van Ooijen 2006) was used both to assign markers to linkage groups and to order markers within linkage groups.
Similar(40)
Then, using these marker dark layers and tephra layers as guides, we further identify thinner but distinct dark layers that can be correlated among the deeper six sites and can be used as second order marker layers.
This option allowed for the definition of fixed order marker subsets based on marker order in the F2 linkage groups.
This widely successful approach relies on recombination to separate and order marker loci.
Prior to ordering, markers with MLOD scores <5 were filtered.
The sorghum genome sequence information was given priority in ordering markers that were indistinguishable genetically.
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