ERAP1 SNPs were genotyped with the TaqMan SNP Made to Order Assays from Applied Biosystems (ABI, Foster City, CA, USA).
So far, we have provided examples that illustrate the indispensable role of histopathology as a first-order assay in discovering gene function.
Combining IVC with microfluidic technology allows monodisperse emulsion droplets to be formed [13], mixed [14], split [15], merged [10], incubated, thermocycled [16], ordered, assayed for fluorescence [17], and sorted [18], all within the confines of a small chip.
To compare the performance of two generally available hybridization-based methods for capturing 56 genes, including exons, introns, and 5 kb of up- and downstream non-coding sequence for massively parallel sequencing, we ordered assays from two companies, Agilent and Roche Nimblegen.
Other instruments measured particles, magnetic fields, and radiation scattered back into space (in order to assay the surface chemistry).
This test was done in order to assay for cytotoxicity by measuring cell proliferation.
Ethanol tolerance test was used in order to assay the ability of the 102 mutations that harboring SPT15 genes.
In order to assay the cytocompatibility and cell behavior on the composite scaffolds, osteoblast cells were seeded on the matrix.
In order to assay the cytocompatibility and cell behavior on the composite scaffolds, fibroblast cells were seeded on the matrix.
The cells were resuspended in medium, and an aliquot was collected in order to assay the amount of internalized radioactivity using the automated gamma counter.
