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LB medium (1 ml) was added and the sample incubated at 37 °C in an orbital incubator (250 rpm) for 1 h.
Then the culture was incubated at 60°C and 150 rpm in an orbital incubator shaker for 96 h.
Cells were incubated for 40 minutes at 35˚C on a Gyromax 737 orbital incubator shaker (Amerex Instruments) at 65 rpm with illumination from a 100-W light bulb to obtain protoplasts.
The mixture was shaken for 2 h in an orbital incubator and the hydrolysis reaction stopped by adding 15 ml CHCl3/CH3OH (2:1 v/v).
Orbital incubator was set at 45 °C and 200 rpm.
Experiment was carried out under anaerobic condition using shaking flasks in an orbital incubator at 32 °C and 150 rpm.
Similar(32)
The reaction mixtures (1 mL) were mechanically shaken in an orbital shaker incubator (Combi-D24 hybridization incubator, FINEPCR®, Yang-Chung, Seoul, Korea) at 50°C.
The reaction mixtures were mechanically shaken in an orbital shaker incubator (Combi-D24 hybridization incubator) at 50°C for up to 72 h.
The reaction mixtures (1 mL) were mechanically shaken in an orbital shaker incubator (Combi-D24 hybridization incubator, FINEPCR®, Yang-Chung, Seoul, Korea) at 50°C in accordance with previously described methods [ 19].
Briefly, 10 14 days after plating of glial cells, 75-cm2 flasks containing confluent cells were rinsed in glial medium and mechanically shaken at 250 rpm for 18 h in an orbital shaker incubator (combi-SV 12 hybridization incubator, Finemould Precision Ind. Co.; Yang-Chun, Seoul, Korea) at 37°C.
Chrysosporium queenslandicum was inoculated into the flask containing 100 ml of BSM with 250 mg of keratinous substrates, and incubated at 28 °C on an orbital shaking incubator (Remi CIS-24 Plus) at 70 rpm for 12 and 24 days.
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