This pattern essentially persisted in larvae, with transcripts detected in the cerebral vesicle, pre-oral pit, club-shaped gland and gill slits (Fig. 2J, K).
These sites correlate with expression of AmphiHh in adjacent tissues, specifically the anterior notochord, central pre-oral pit and pharynx [6].
Hh/Gli signalling has been reported to be required for vertebrate pituitary development [68], [69], and hence the expression of AmphiHh and AmphiGli in the developing pre-oral pit may reflect a conserved role.
The pre-oral pit of amphioxus has been suggested to be homologous to the vertebrate pituitary on the basis of embryological and gene expression data, and due to its post-metamorphic fate, when it establishes a connection to the ventral cerebral vesicle [64] [67].
We also detected AmphiGli transcripts in the developing left head cavity towards the end of neurulation, and in the developing cerebral vesicle, pre-oral pit (which forms in part from the left head cavity) and endoderm associated with the developing club-shaped gland and gill slits.
In the larva, expression is observed in the club-shaped gland, in the pre-oral pit, around the mouth and in the most anterior and dorsal part of the pharyngeal endoderm.
Each pit opens to the exterior through anterior and posterior nares; there is no connection with the oral cavity.
Sites included the surface ectoderm lining the branchial arches and limb buds, oral ectoderm, nasal pits, otic vesicles and Rathke's pouch (Fig. 4C F; Supplementary Material, Fig. S5).
He pulled out a camera and forced me to give his pit bull oral sex while he videotaped it.
Mooting competitions, which see competitors pitting their oral presentation skills against peers from across the country and even around the world, give students the chance to develop their persuasion skills to a high level.
Bret snorts five grams of cocaine while the pit bull performs oral sex on him.
