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Specific mRNAs were semiquantitated via reverse transcription (RT-PCR) or real-time PCR with the iCycler IQ detection system Bio-Rad Laboratoriess, Hercules, CA) using SYBR green I (Molecular Probe, Eugene, OR) in triplicate reactions.
Analyses were performed either in duplicate or in triplicate in independent assays.
Each methylation analysis was performed in duplicate (for CD133+ versus CD133- samples) or in triplicate (for all other methylome analyses).
All experiments were performed in duplicate for the caspase-Glo 9 assay or in triplicate for the caspase-Glo 3/7 assay.
A total of 7,680 cDNA (4,938 distinct entries) were successfully amplified by PCR from plasmids and spotted on the array once, twice or in triplicate.
Similar(55)
All measurements were done in duplicate (PAB046) or in triplicates (PAB036, PAB050, WT-ABD) and averaged.
All activity assays were performed either in duplicates or in triplicates.
The cells were cultured (37°C, 5% CO2) in duplicates in 24-well (for culture supernatant), or in triplicates in 96-well (for proliferation assay), flat-bottom tissue culture plates.
Briefly, cells cultured in six-well plates were infected with RB51 or S2308 in triplicate as described above.
Baseline expression was established by mock-treated cells with the same volume of acetic acid or ethanol in triplicate.
They were incubated with various concentrations of NiCl2, CoCl2, or CrCl2 in triplicate.
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