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Both vector systems encode two different affinity tags, an upstream albumin binding protein and a downstream hexahistidyl peptide, and are furnished with cleavage sites for two site-specific proteases for optional affinity tag removal.
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These high-affinity and conserved sites serve as nucleation centers, or "seeds", to govern the proper assembly of TFs at one promoter, which also involves a set of additional transcription factors with binding sites of decreasing affinity and acting in a concomitantly optional manner.
There's a bunch of optional stuff you can do during the game that boosts your "affinity" with specific characters, but you're never given any idea of what affinity actually does.
The hydrogen bond interactions to the serines in TM5 may, however, contribute to agonist affinity, so the Ser-TM5 feature was therefore defined as optional in the new pharmacophore.
The result is two-fold: the His-tag facilitates purification by immobilised metal affinity chromatography, whilst the fusion domains act primarily as solubility aids during expression, in addition to providing an optional purification step.
Sideburns optional.
Eat (optional).
Affinity indeed.
Intelligence optional.
Wear: Optional.
Handcuffs optional.
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