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A two-stage optimization scheme was applied where one maximum was found and the optimization process was then re-initialized from the current optimum until the objective-function value did not change appreciably between consecutive re-initializations, or a predefined number of re-initializations was reached.
As can be seen for the trade-off boundary drawn, there is a wide range of virulence associated with each step of evolution toward the optimum, until the optimum is neared (depending on the shape of the trade-off).
In all cases where a single or a multidrug solution is found, also all suboptimal solutions are hierarchically identified, iterating the procedure while excluding the current optimum, until the problem becomes unfeasible (i.e. no more solutions exist, capable of blocking that objective reaction).
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We conducted several "Curve fitting"s with the default of 40 iterations, where each "Curve fitting" started with the previously estimated optimum values until no improvement was observed.
E. coli C cells were grown at 37°C for the optimum temperature until a concentration of 2 × 10 CFU/ml was reached as determined by OD600.
It was then spread uniformly over a surface sterilized (70%% ethanol) plastic sheet until optimum moisture (51 54 %) is attained.
Here, it can be seen that the ecological function, exergy destruction, and cooling load increase logarithmically until optimum point with the increase of the aR parameter.
Similarly, Fig. 4b shows that in the first 20 min, the percentage removal of AA from aqueous solution increased rapidly from 2 to 15% and decreased thereafter which then increased slowly until optimum time was reached at 120 min for the adsorbate and subsequently became constant.
Finally, the membranes were washed five times with TBS-T for 5 min and developed with 3,3'-diaminobenzidine (DAB, Sigma) substrate until optimum color development was observed.
Finally, the plates were developed with OPD buffer (Sigma) until optimum development occurred, when the reaction was stopped with 3 M sulphuric acid prior to spectrophotometric reading at 490 nm.
Finally the plates were developed with OPD buffer until optimum development occurred and the reaction was stopped with 3 M sulphuric acid prior to spectrophotometric reading at 490 nm.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com