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The equation for optimum flow rate is derived.
S.P. identified the source of cells and helped in establishing protocol for improved medium, optimum flow rate, cell density and MTT assay.
Thus, an optimum flow rate of 50 µl/min for growing and maintaining all three cell types was chosen for all subsequent experiments (Fig. 2A for schematic representation).
The optimum flow rate per inlet for a stable profile was also determined.
The scaffolding system for each cell type and the optimum flow rate to culture and maintain the three cell types under the same conditions were established as a priority aim.
P.M.A. constructed the multicellular co-culture flow system, developed improved medium, optimum flow rate and cell density, conducted immunocytological and MTT assay experiments, data analysis and drafted the manuscript.
Similar(35)
The computer model simulates the thermodynamic and heat transfer processes where a range of optimum flow rates are determined.
Based on one at a time method, the optimum flow rates of H2, air and N2 were 375, 259 and 2.6 mL min-1, respectively.
More specifically, it is interesting that there is optimum mass flow rate and optimum fin length.
The optimum collector area for the present application is 300 m2, the optimum collector flow rate is 54 kg/m2 h and the optimum storage tank size is 25 m3.
It was found that the optimum gas flow rate was 6 L/min whereas the optimum pH value is 6.5.
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