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This approach typically results in poorly expressed recombinant protein since the wild-type foreign genes have not been evolved for optimum expression in the host.
Two variants of the haemagglutinin (HA) surface glycoprotein gene were synthesised for optimum expression in plants: these were the full-length HA gene (H5) and a truncated form lacking the transmembrane domain (H5tr).
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The results of the study suggest that buccal mucosa may indeed be useful for factors selected carefully for optimum expression change in buccal tissue.
Codon optimization, a genetic technique used to achieve optimum expression of a foreign gene in a host's cell system is achieved by replacing existing codons of a specie with a set of more suitable host codons [31] [34].
To obtain optimum expression of foreign RparsM gene in the P. putida KT2440 strain, we compared the codon usages of RparsM to P. putida.
The purpose of this review is to familiarize the researcher with the biological principles involved in the formation of disulfide-bonded proteins with the hope of guiding the scientist in choosing the optimum expression system.
The novel recombinant Anti-CD22 scFv apoptin protein was expressed in E.coli BL 21 DE3) using the pET expression vector driven by the strong T7 promoter under optimum expression condition.
The optimum expression level will be intermediate, at the point where the marginal benefit of protein function equals the cost of additional expression (the vertical line in fig. 1).
Our results also demonstrate that some chemokines require the activation of p38-MAPK for optimum expression.
Because the MMP expression levels were highly skewed, Box-Cox transformations [ 15] were used for the determination of p-values, with optimum λ = 0,19 for MMP1 in tumor stroma and optimum λ = 0,16 for MMP1 expression in tumor cells.
It could be observed that the optimum power allocation expressions in (38) and (39) at high SNR regimes mainly depend on the value of noise variances.
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