Sentence examples for optimum assay from inspiring English sources

Different parameters, such as the amount of immunoreagents, type of the materials, composition of the blocking solution and of the detector reagent mixture, were investigated to provide the optimum assay performance.

The optimum assay conditions were: methanol acetonitrile dipotassium hydrogen phosphate (pH 7.0; 15.3 mM) (20 33 47, v/v/v) as the mobile phase and at a flow rate of 1.19 ml/min.

The optimum assay conditions were: methanol/acetonitrile-sodium pentane sulfonate (pH 2.2; 7.5 mM) (33.4 66.6, v/v) at a mobile phase flow rate of 1 ml/min during a 40.6 min gradient time.

The predicted optimum assay condition consisted of methanol and potassium dihydrogen phosphate buffer (pH 3.2; 25 mM, 0.5% Triethylamine) in a proportion of 60 40% v/v, respectively, as the mobile phase at a flow rate of 1.2 mL min−1.

Experiments were performed to empirically determine an optimum assay and elution procedure (data not shown).

To determine optimum assay conditions, several experiments were performed.

Temperature optimum assays were performed in HEPES (pH 8) buffer at 4, 12, 25, 37, 42, 50 and 65°C.

The results were expressed as relative activity to the value obtained at either optimum temperature assay or optimum pH assay.

The pH optimum was assayed by measuring enzyme activities at different pH levels (Figure 5A).

For pH optimum evaluation, assays were carried out using MES (pH 5.5, 6.0) MOPSO (pH 6.5, 7.0, 7.5) or Bis-tris propane (pH 8.0 to 9.5).

The interacting mechanism was studied and the optimum conditions for assay of PY were also investigated.