Despite efforts to optimize the methodology, misleading results are still possible, even when standard optimization approaches are followed.
This study optimizes the methodology to obtain micro-, submicro- and nanoencapsulation structures based on zein (a maize protein) and dodecane (a PCM paraffin which has a transition temperature at −10 °C).
Further work to optimize the methodology may reduce the measurement error.
KS optimized the methodology for preparation of 2-DE gels and prepared the gels.
There are several ways to deal with the generation of antivector antibody but further investigations are required to optimize the methodology.
We optimized the methodology in mammalian cells by using an engineered Tyr-RS, and a chimera of human and Bacillus stearothermophilus tRNATyr to heterologously express low-abundance GPCRs.
RL participated in the conception and design, developed and optimized the methodology, acquired the data, analysed and interpreted the results, drafted the manuscript and gave final approval of the version to be published.
Although higher purification factors have been reported for penicillin acylase [ 45], the main objective of this study was not to optimize the methodology, but rather to compare the performance of the purification process when handling cell lysates derived from cells cultivated and induced under different conditions.
To optimize the methodology, SF samples were tested at different dilutions (1 5, 1 10, 1 100, and 1 1000) before and after enzymatic digestion with hyaluronidase (50 units/ml), with chondroitinase ABC (0.1 units/ml), or with a combination of both enzymes at 37°C for 90 minutes.
The ionic conductivity at room temperature improved from 6.6×× 10−5 S/cm to 1.4 × 10−3 S/cm by optimizing the processing methodology and the lithium ion concentration.
