These modifications were introduced to make the SGM formulation more similar to that of NGM, and to optimize the media for optimal fermentation performance of laboratory strains (Harsch et al. [2010]; Rossouw and Bauer [2009]).
The response surface methodology (RS M -central composite design (CCD) was performed to optimize the media for biosurfactant production.
Statistics based optimization, Plackett Burman design (PBD) and response surface methodology (RSM) were employed to screen and optimize the media components for the production of naringinase from Aspergillus brasiliensis MTCC 1344, using solid state fermentation.
A sequential optimization based on statistical design and one-factor-at-a-time (OFAT) method was employed to optimize the media constituents for the improvement of citric acid production from oil palm empty fruit bunches (EFB) through solid state bioconversion using Aspergillus niger IBO-103MNB.
A potential strategy that shall be further investigated is to optimize the media to provide nitrogen and phosphorus in a way that enables a one-step process without the need to exchange the media.
The various codecs exist to optimize the media streaming based on the application requirements and network bandwidth; some of the implementations rely on narrowband and compressed speech, while others support high-fidelity stereo codecs.
RSM based on CCD was also used to optimize the media for cellulase production using four medium constituents, OPEFB fibers (5 15 g/L), yeast extract (3 9 g/L), CaCl2 (1–5 mM), MgSO4 (3–7 mM) and to optimize the level of two environmental condition agitation speed (200 300 rpm) and temperature (28 32 °C) from Aspergillus terreus [29].
In order to optimize the media for F420 production, the ZY component of ZYM 5052 media was replaced by commonly used media bases including 2× ZY, YT (0.8% tryptone, 0.5% yeast extract and 42.77 mM NaCl), TB (1.2% tryptone, 2.4% yeast extract and 0.4% glycerol), SOB (2% tryptone, 0.5% yeast extract, 8.56 mM NaCl, 2.5 mM KCl and 10 mM MgCl2) and SOC (SOB with 20 mM glucose).
However, in order to observe substantial differences in fxo between the strains, it is necessary to optimize the media conductivity (σm) using the strain-types that exhibit maximum differences in their cell wall roughness.
A remarkable amount of research and resources have been expended towards optimizing the protocols, freezing media composition, cooling devices and storage containers, as well as developing good manufacturing practices in order to ensure that MSCs retain their therapeutic characteristics following cryopreservation and that they are safe for clinical use.
