Delivery of a vaccine to a mucosal surface optimizes the induction of mucosal immunity.
To optimize the induction of CD8+ T cell responses, we made several modifications to NP, aiming at retaining the protein in the cytosol or targeting it to the proteasome.
To optimize the induction of catabolic proteases (that is, MMP-13, ADAMTS family) by IL-1 in both primary and immortalized chondrocytes, we used serum-free media for the duration of the treatment period as in a previous study [ 44].
Vacuum pressure, vacuum period, root wounding and bacterium suspension density were selected to optimize the induction of transient expressing plants by using the vacuum desiccator SHZ-D Ⅲ (Yuhua Inc., Henan, China).
In this study, we improved the structure of inclusion bodies (IBs) by optimizing the induction temperature, pH, concentrations of inducer, and metal ion additives.
The use of statistically based experimental design is a vital tool in optimizing the induction conditions that may bring several fold increment in bromelain production.
Hence, we optimized the induction coils based on the requirements of compact size and low noise level.
Several one-factor experiments and an orthogonal test (L 9)(3)(4)) were employed to optimize the induction conditions.
To optimize the induction condition, samples from various fractions including inclusion body, cytoplasm, periplasmic space, and culture supernatant taken at various time points after induction with different concentration of IPTG were analyzed by SDS-PAGE activityvity assay.
Wrapper methods measure the usefulness of feature subsets according to their predictive power, optimizing the subsequent induction procedure that uses the respective subset for classification [51, 60, 61, 62, 63].
