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Optimizing the expression of efflux transport in biocatalyst cells can lead to increased product yield, improved product tolerance of production organisms, and maximized product recovery from the extracellular medium.
If a language exposes a simple to grasp MoC in a consistent way across architectures, the programmer can concentrate the efforts on optimizing the expression of parallelism in the application instead of porting code to a given system.
In this paper, we significantly increased the limonene yield in E. coli by optimizing the expression of the two limonene biosynthesis genes, overexpression of the two rate-limiting enzymes in the endogenous MEP pathway, and optimization of the culture medium (Figure 6).
After optimizing the expression of these chemokine receptors, we proceeded to systematically screen 96 detergents for use in purification procedures.
The plasmids, mouse ESCs (mESCs), expression cassettes and findings that we describe should be of general use in generating other LCA alleles, basal exchange cassettes, and for optimizing the expression of FPs and other reporter alleles in mESCs and mice.
This multiple pathway engineering approach that focuses on optimizing the expression of several related pathways is potentially useful for gene manipulation that can be directly applied to engineering microalgal fatty acid production.
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A series of modifications to optimize the expression of those enzymes and the medium have been done.
A number of vectors are being designed to optimize the expression of transgenes.
With additional background genome selection, the recurrent genome background can further improve the %RGC and optimize the expression of introgressed QTLs.
Therefore, there will be significant opportunities for agronomists and farmers to use their skill to optimize the expression of the root potential of the selected varieties under various conditions.
To obtain the highly efficient whole-cell biocatalyst, five expression plasmids were constructed to optimize the expression of 5-aminovaleramide amidohydrolase and l-lysine 2-monooxygenase in Escherichia coli.
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