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Comparing directly to clonogenic survival analysis, they showed that after optimizing for cell number and allowing for a sufficient number of cell doublings after irradiation constructing survival curves based on cell number (a nonclonogenic assay) provides an accurate measure of radiosensitivity and, moreover, allows for the identification of radioresponse modifiers.
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The matrices were fabricated, biophysically characterized and optimized for cell culture applications.
For example, single cells actively migrating might be upregulating expression of genes and proteins optimized for cell locomotion, while those establishing cell-cell attachment are upregulating expression of adhesion molecules such as E-cadherin necessary for intercellular junction organization.
Our results suggest the yeast cells have been optimized for cell polarity in spatial gradients.
For example, the surface of the polymer gel can be optimized for cell attachment.
A sandwich ELISA was designed and optimized for cell lysates based on previously published methods (51– 51).
The lifetime of the epithelium might be shorter under BAK conditions whereby not all of the parameters can be optimized for cell performance.
Although the amount of yeast extract optimized for cell growth is small (0.79 g/L), excluding yeast extract from the medium would reduce growth significantly (unpublished observations).
NCS patient fibroblasts were transfected by nucleofection using the Amaxa® Human Dermal Fibroblast Nucleofector® Kit (Lonza) and a Nucleofector 2b NHDF program optimized for cell viability following nucleofection.
Interestingly, Fas is expressed at lower levels on some human acute leukemia progenitor cells, making attractive the use of a potent Fas agonist optimized for inducing cell death and able to fully eradicate hematopoietic cancers.
However, primary cells of different origins respond differently, and methods need to be carefully optimized for each cell type.
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