Sentence examples for optimizing expression from inspiring English sources
Finally, by optimizing expression of hmaS, hmo and hpgT and attenuation of l-phenylalanine transamination, the l-Phg yield was increased by 224-fold comparing with the original strain.
Fine-tuned gene knockdown by controlling sRNA abundance will be useful for rapid design of microbial strains through simultaneously optimizing expression of multiple genes at a systems level, as it overcomes the difficulties of constructing and testing many different sRNAs and checking their cross-reactivity.
Optimizing expression is also important, and coregulating a xylose isomerase with downstream enzymes may prove effective.
By optimizing expression vectors and expression conditions, all BVMOs were obtained in a soluble form.
As for construction of microbial cell factory, improving and optimizing expression levels of the enzymes in the metabolic pathway was always an important strategy; however, concentrations of specific enzymes could not be endlessly increased.
Overall, all of these relatively new approaches are based on spatial, temporal, and quantitative regulation of gene expression at the transcriptional or translational level, or both, thereby enabling production of higher amounts of specialized metabolites by overcoming bottlenecks, optimizing expression of genes, and redirecting the flux of precursors.
To optimize expression, two codon-optimized coding regions have been designed and expressed alongside the wild-type coding region.
SDS PAGE analysis was carried out to check protein size and to check and optimize expression.
We therefore re-engineered the TFIIE subunit production vectors and tested various Escherichia coli host strains to optimize expression.
Further experiments were conducted using pFab3 to optimize expression levels using different strains of E. coli and various culture conditions.
The codons for the 2A and 1D regions were adapted to the codon usage of S. cerevisiae to optimize expression in yeasts (Fig. 1).
